一、2020年，西北农林科技大学科技学院动物遗传育种与繁殖重点实验室发表标题为：A Potential Nutraceutical Candidate Lactucin Inhibits Adipogenesis through Downregulation of JAK2/STAT3 Signaling Pathway-Mediated Mitotic Clonal Expansion的文章到CELLS已见刊；

二、使用Zeta Life胎牛血清Z7186FBS-500培养 3T3-L1小鼠胚胎成纤维细胞(前脂肪细胞)；

三、文章简介：乳糖酶抑制剂 通过下调脂肪形成 JAK2 / STAT3信号通路介导的有丝分裂.

四、文章部分内容见下：

Cell Culture and Adipocyte Differentiation Induction

3T3-L1 preadipocytes obtained from American Type Culture Collection (ATCC) were maintained in DMEM (SH30022.01, HyClone, CT, USA) containing 10% fetal bovine serum (Z7186FBS-500, ZETA LIFE, Menlo Park, CA, USA). To induce differentiation, 2-day post confluent 3T3-L1 cells (designatedat day 0) were cultured in DMEM containing 10% FBS, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX)(I7018, Sigma-Aldrich, St. Louis, MO, USA), 1 µM dexamethasone (D4902, Sigma-Aldrich, MO, USA),and 10 µg/mL insulin (91077C, Sigma-Aldrich, St. Louis, MO, USA) for two days. Cells were thenmaintained in DMEM containing 10% FBS and 10 µg/mL insulin for the rest of the culture.

产品名称

货号

规格

Fetal Bovine Serum France Origin

Z7186FBS-500

500ml

Fetal Bovine Serum France Origin

Z7186FBS-100

100ml

Fetal Bovine Serum ,South America Origin

Z7181FBS-500

500ml

Fetal Bovine Serum ,South America Origin

Z7181FBS-100

100ml

Fetal Bovine Serum (South America Origin) Ultra-low Endotoxin

Z7180FBS-500

500ml

Fetal Bovine Serum (South America Origin) Ultra-low Endotoxin

Z7180FBS-100

100ml

Fetal Bovine Serum (Australia Origin）

Z7010FBS-500

500ml

Fetal Bovine Serum (Australia Origin）

Z7010FBS-100

100ml

Fetal Bovine Serum ,South America Origin

Z7185FBS-500

500ml

Fetal Bovine Serum ,South America Origin

Z7185FBS-100

100ml

Zeta Life介绍

美国 Zeta Life 公司成立于 1989 年，是无血清细胞培养基 DMEM/F12 主要完成人，有三十多年的胎牛血清、无血清细胞培养基、以及干细胞、免疫细胞及肿瘤细胞等多种不同类型哺乳动物细胞的无血清培养液生产经验；也是目前世界胎牛血清、无血清培养基较大的 OEM 供应商；每批血清均有完整的血源证明文件、兽医师检验证明及品质测试报告；ZETA LIFE 血清在 100 级的无菌间采用全自动方式制造生产，具有良好的可再现性及可追溯性。

2018 年美国 Zeta Life 公司与美国加利福尼亚大学旧金山校区联合开发活细胞、活体动物体内可代谢的 Advanced DNA RNA 转染试剂，此技术成为蛋白功能、免疫细胞及干细胞治疗、研发及生产的主要关键技术